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Structure function relationship of glycosaminoglycans

Glycomics Approach to Structure-function Relationships of

Glycosaminoglycans (GAGs) are naturally occurring polysaccharides, composed of alternating sugar units; these include an into the GAG structure-function relationships. In parallel, metabolic engineering tools, knock-in/out animal models and mam-malian cell culture models, have also contributed to deciphering the GAG biosynthetic pathway and. Glycosaminoglycans (GAGs) or mucopolysaccharides are long linear polysaccharides consisting of repeating disaccharide units (i.e. two-sugar units). The repeating two-sugar unit consists of a uronic sugar and an amino sugar, with the exception of keratan, where in the place of the uronic sugar it has galactose THE JOURNAL OF B~LOGICAL CHEMISTRY 0 1990 by The American Society for Biochemistry and Molecular Biology, Inc. Vol. 265, No. 36, Issue of December 25, pp. 223.86-22391.1990 Printed in U.S A. Glycosaminoglycan-mediated STRUCTURE-FUNCTION RELATIONSHIPS* Leuserpin-2/Thrombin Interaction (Received for publication, June 18, 1990) Hermann From. Glycosaminoglycans (GAGs) are a class of these polysaccharides, which bind to a wide variety of proteins and signaling molecules in the cellular environment and modulate their activity, thus impinging on fundamental biological processes

Connective tissue matrix contains the linear polysaccharide (glycosaminoglycan) hyaluronan, and the glycosaminoglycans chondroitin / dermatan sulfate, and keratan sulfate covalently linked to protein (proteoglycans) Glycosaminoglycans (GAGs) are primary components of the cell surface and the cell–extracellular matrix (ECM) interface. Advances in the technology to analyze GAGs and in whole-organism genetics have led to a dramatic increase in the known important biological role of these complex polysaccharides Glycosaminoglycan structures have a high degree of heterogeneity (termed fine structure) with respect to molecular weight, disaccharide construction, and sulfation. This variability can be attributed to the fact that glycosaminoglycan synthesis is dynamic Different glycosaminoglycans were tested to evaluate their effects on proliferation and differentiation processes of a monoblastic leukemia cell line (U-937). Heparin and derivatives (from 0.1 to 100 μg/ml) inhibit cell proliferation; heparan sulfate does not produce modifications, while chondroitin sulfate and dermatan sulfate (from 0.01 to.

The full text of this article hosted at iucr.org is unavailable due to technical difficulties Read Effects of glycosaminoglycans on proliferation of epithelial and fibroblast human malignant mesothelioma cells: a structure-function relationship, Cell Proliferation on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips A chemical approach to elucidating the structure-function relationships of chondronitin sulfate glycosaminoglycans Hsieh-Wilson, Linda C. / California Institute of Technology: NIH 2017 R01 GM: A chemical approach to elucidating the structure-function relationships of chondronitin sulfate glycosaminoglycans

This model establishes the conceptual framework for a new class of computational tools to use to assess patterns of domain organization within glycosaminoglycans. These tools will provide a means to consider high-level chain organization in deciphering the structure-function relationships of polysaccharides in biology They are widely distributed as glycosaminoglycans (GAGs) sidechains of proteoglycans (PGs) in the extracellular matrix and at cellular level. (OA), this increased understanding of GAG structure-function relationship has led to the discovery of novel pharmaceuticals for the possible treatment of serious diseases, such as cancer. In this. Glycosaminoglycans (GAGs) contribute to myriad, and very often opposing, functions such as cellular proliferation as well as apoptosis, self-renewal as well as differentiation. Yet, precious little is understood about the exact GAG sequences that induce these functions Structure-Function relationships of equine menisci. PLoS One. to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties. The meniscus architecture was investigated histologically. (Glycosaminoglycans) 9007-34-5. 2. Structure-function of major ECM proteins of valvular tissue. Proteins are the most abundant organic components of the human body. There are roughly 100,000 different kinds of proteins and they account for about 20% of the total body weight [].All proteins contain carbon, hydrogen, oxygen, and nitrogen and in some cases small quantities of sulfur

Effects of glycosaminoglycans on U-937 leukemia cell

  1. Study Cell Organelles - Structure-Function Relationship flashcards from Rachel A's QMUL class online, or in Brainscape's iPhone or Android app. Learn faster with spaced repetition
  2. oglycans (GAGs) is very scanty. In fact, to date, there is only one study available on the evaluation of human milk GAGs ( Newburg et al. 1995 ) and no data exist on bovine milk, which is commonly used for the preparation of infant formulas
  3. neered the tissue's structure function relationships must be better understood. Currently, TMJ disc structure function relationships are described only in terms of its most abundant biochemical element, collagen. At !80 90% of the dry weight [6,7], collagen is the major structural component of the disc, and its anisotropy an
  4. oglycans content. Thus, a deep understanding of the relationship between SPs' structural characteristic and bioactivities is still ugently needed.
  5. Understanding structure-function relationships in the temporomandibular joint (TMJ) disc is a critical first step toward creating functional tissue replacements for the large population of patients suffering from TMJ disc disorders. While many of these relationships have been identified for the collagenous fraction of the disc, this same.
  6. oglycans are complex polysaccharides, which play major biological roles in health and disease, intracellularly, at O-sulfation in the structure/function relationships of heparan sulfate, its synthesis by 6-O-sulfotransferases, and its remova
  7. oglycans remains a challenge but is essential for deter

Figure-2- The acid sugars present in Glycosaminoglycans are D- Glucuronate, and L- Iduronate. Structure-function relationship. Because of their large number of negative charges, these heteropolysaccharides chains tend to be extended in solution. They repel each other and are surrounded by a shell of water molecules Effects of glycosaminoglycans on U-937 leukemia cell proliferation and differentiation: structure-function relationship. Volpi N, Petrini M, Conte A, Valentini P, Venturelli T, Bolognani L, Ronca G. Exp Cell Res, 215(1):119-130, 01 Nov 1994 Cited by: 13 articles | PMID: 795766 Although glycosaminoglycans contribute to diverse physiological processes1,2,3,4, an understanding of their molecular mechanisms has been hampered by the inability to access homogeneous. primarily comprised of collagen, elastic fibers, and proteoglycans and glycosaminoglycans, although other ECM components are present as well. Taken together, the ECM performs several roles in heart valves. First, the ECM plays a biomechanical role: it is responsible fo

Recognizing the need to take an integrated approach to advance glycan structure-function relationships, several international collaborative efforts, namely the Consortium for Functional Glycomics. Fractions and fragments of heparins and heparin-derived oligosaccharides were obtained to investigate the metabolism and the structure function relationship with plasma and cellular proteins. Binding of heparin and heparin fragments to human endothelial cells was demonstrated using radiolabeled compounds (4)

The glycosaminoglycan (GAG) class of carbohydrates is characterized by unparalleled sequence diversity. Located on the cell surface and in the extracellular matrix, specific structural features are required for a variety of functions including coagulation, cell-signaling and wound healing among others The regular polymer sequence of glycosaminoglycans is a result of the repetitive units of glycosaminoglycan chains. not to replace the relationship between patient and physician/doctor and the. glycosaminoglycans and proteoglycans provide the water-loving space filling component. • Each tissue has a specialized ECM to support form and function. • Factors that influence ECM structure and function include, ECM composition, cellular organization, and cross-linking The fact that sulfated glycosaminoglycans (GAGs) are necessary for the functioning of all animal physiological systems drives the need to understand their biology. This understanding is limited, however, by the heterogeneous nature of GAG chains and their dynamic spatial and temporal expression patterns. GAGs have a regulated structure overlaid by heterogeneity but lack the detail necessary to.

Effects of glycosaminoglycans on proliferation of

Recent progress in coupling bacterial GAG-degrading enzymes with sensitive analytical techniques has led to a revolution in understanding the structure-function relationship for an important subset of GAGs, namely heparin/heparan sulfate-like glycosaminoglycans (HSGAGs) The specific sequence and chemical organization of these polymers likely define function; however, identification of the structure-function relationships of glycosaminoglycans has been met with challenges associated with the unique level of complexity and the nontemplate-driven biosynthesis of these biopolymers

The Structure of Glycosaminoglycans and their Interactions

Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans. We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma. Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components that contain two structural parts with distinct functions: a protein core and glycosaminoglycan (GAG) side chains. CSPGs are known to be involved in important cell processes like cell adhesion and growth, receptor binding, or cell migration. It is recognized that the presence of CSPGs is critical in neuronal growth. Structural characterization of glycosaminoglycans remains a challenge but is essential for determining structure-function relationships between glycosaminoglycans and the biomolecules with which they interact and for gaining insight into the biosynthesis of glycosaminoglycans. We have recently reported that xyloside-primed chondroitin/dermatan sulfate derived from a human breast carcinoma cell.

Metabolism and Structure-Function Relationships of

The overall focus of our research is to characterize and eventually to manipulate the structure-function-environment relationship of soft tissues in an integrative fashion (cellular, tissue, organ, and clinical). (especially glycosaminoglycans (GAGs) and proteoglycans) of these tissues. These techniques have allowed us to gain understanding. Alpha- and beta-linked 1,3-glucans have been subjected to conversion with p-toluenesulfonic acid (tosyl) chloride and triethylamine under homogeneous reaction conditions in N,N-dimethyl acetamide/LiCl.Samples with a degree of substitution of tosyl groups (DS Ts) of up to 1.91 were prepared by applying 5 mol reagent per mole repeating unit.Hence, the reactivity of α-1,3-glucan is comparable. Glycosaminoglycans (GAGs) are linear polysaccharides found in a variety of organisms. GAGs contribute to biochemical pathway regulation, cell signaling, and disease progression. GAG sequence information is imperative for determining structure-function relationships

Glycosaminoglycans: molecular properties, protein

Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients. Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn. Biomechanical properties of human menisci are site- and depth- specific Structure-function relationships in soft tissue mechanics: Examining how the micro-scale architecture of biochemical constituents effects health Also, they are made of strikingly similar constituents, primarily consisting of water, type I collagen, glycosaminoglycans and elastin. The microstructure of these tissues, however, is very. In addition to the pathogenesis of OA, cartilage structure-function relationships have attracted intense research [Kempson et al., 1976;Armstrong and Mow, 1982;Setton et al., 1993]. The earliest signs of OA seem to involve changes in the composition and structure of the superficial cartilage [Buckwalter and Mankin, 1997] Glycosaminoglycans are linear, anionic polysaccharides (GAGs) consisting of repeating disaccharides. Indeed, most GAG chains are formed from repeating disaccharide units of hexosamine and hexuronic acid. Such features shed light on biological processes and GAG structure/ function relationships. We invite investigators to submit reviews or.

The specific sequence and chemical organization of these polymers likely define function; however, identification of the structure-function relationships of glycosaminoglycans has been met with challenges associated with the unique level of complexity and the nontemplate-driven biosynthesis of these biopolymers.To address these challenges, we. the structure/function relationship of its relevant isomerase and dehydrogenase. Two proteins (gbs1892 and gbs1891) of Strepto-coccus agalactiae strain NEM316 were overexpressed in Esche- glycosaminoglycans are bound to core proteins as a proteogly-can (5) Different glycosaminoglycans were tested to evaluate their effects on proliferation and differentiation processes of a monoblastic leukemia cell line (U-937). Heparin and derivatives (from 0.1 to 100 micrograms/ml) inhibit cell proliferation; heparan sulfate does not produce modifications, while chondroitin sulfate and dermatan sulfate (from 0. In BriefGlycosaminoglycans (GAGs) remain one of the most challenging posttranslational modifications to study, much due to their structural complexity and heterogeneity, and new methods for analysis are therefore required. We have developed a protocol for enrichment and structural characterization of GAGs of proteoglycans using nLC-MS/MS. We provide detailed information on the nonreducing end.

Abstract. Heparin and heparan sulfate (Hp/HS) are linear complex glycosaminoglycans which are involved in diverse biological processes. The structural complexity brings difficulties in separation, making the study of structure-function relationships challenging The sulfation at the 3-OH position of glucosamine is an important modification in forming structural domains for heparan sulfate to enable its biological functions. Seven 3-O-sulfotransferase isoforms in the human genome are involved in the biosynthesis of 3-O-sulfated heparan sulfate. As a rare modification present in heparan sulfate, the availability of 3-O-sulfated oligosaccharides is very.

Heparin and heparan sulfate (Hp/HS) are linear complex glycosaminoglycans which are involved in diverse biological processes. The structural complexity brings difficulties in separation, making the study of structure-function relationships challenging. Here we present a separation method for Hp/HS oligosaccharide fractionation with cross-compatible solvent and conditions, combining size. •Developed high-throughput, enzyme-coupled fluorescence assay to identify the first cell-permeable, small-molecule inhibitors of sulfotransferase these structural-functional relationships have been extensively explored during brain development. Structure Although many of these features are common to all PGs, six distinct classes of GAG chains are differenti-ated on the bases of monosaccharide composition, sulfa-tion pattern and other postsynthesis modifications, fo The alternative splicing of the cxcl12 gene generates a recently identified isoform, CXCL12c, which structure/function relationships remain unexplored. The high occurrence of basic residues that characterize this isoform suggests however that it could feature specific regulation by HS. Methodology/Principal Findings

Hyaluronic Acid & Hyaluronidase - YouTube

The temporomandibular joint (TMJ) disc is a little understood structure that, unfortunately, exhibits a plethora of pathologic disorders. Tissue engineering approaches may be warranted to address TMJ disc pathophysiology, but first a clear understanding of structure-function relationships needs to be developed, especially as they relate to the regenerative potential of the tissue These studies, spanning the years of 1988-1996, in which structure/function relationships were the primary focus of several investigators, have been summarized in several reviews (17- 19). Structural studies were undertaken that determined the relative affinities and IGF binding capacity of each form of IGFBP for IGF-I and IGF-II ( 20 , 21 ) The role of a cell-surface receptor in the mechanisms of action of PEDF represents a key aspect of regulation. Along these lines, our studies focus on structure-function relationships and enzymatic characterization of PEDF receptors, and their distribution in the normal and diseased retina Research Strategy. The Sasisekharan Lab rests on a foundation of diverse glycomics technologies developed and applied over the years. We have tackled underlying problems in glycomics and focus on the fundamental aspects of glycan-protein interactions. Bringing various approaches to bear on these problems has been a principal area of focus for.

Material properties and structure-function relationships in the menisci. Clin Orthop Relat Res 19-31, 1990. ISI | Google Scholar; 15. Gabrion A , Aimedieu P , Laya Z , Havet E , Mertl P , Grebe R , Laude M. Relationship between ultrastructure and biomechanical properties of the knee meniscus. Surg Radiol Anat 27: 507-510, 2005 However, natural GAGs are structurally complex and heterogeneous, making structure-function relationships hard to determine and clinical translation difficult. Importantly, subfractions of GAGs with specific chain lengths and sulfation patterns have been shown to activate key signalling processes during stem cell differentiation The main approach of the Heart Valve Laboratory is to study the structure/function relationship of heart valve tissues so that we can determine the failure mechanisms and find ways to improve the design and fabrication of existing artificial heart valves. Our ultimate goal is to develop a bioprosthetic valve that completely mimics the function. (1) characterized impaired structure-function relationship in injury synovial fluid using a cartilage on cartilage mechanical friction test and characterization of protein and glycosaminoglycan.

Biomechanics G&R Lab

Effects of glycosaminoglycans on cell proliferation of normal osteoblasts and human osteosarcoma cells depend on their type and fine chemical compositions Find papers alphabetically by title. Effects of glycerol additions on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectan Gene name - division abnormally delayed and dally-like Synonyms - . Cytological map position - 66E1--66E2 and 70E5--7 Function - growth factor binding protein Keywords - wing, optic lobe, Decapentaplegic signaling Symbol - dally and dlp FlyBase ID: FBgn0263930 and FBgn0041604 Genetic map position - Classification - glypican related proteoglyca structure-function relationship. 1. Introduction. Jridi et al. (2018) found the high bioactivities of SPs might be due to glycosaminoglycans content. Thus, a deep understanding of the relationship between SPs' structural characteristic and bioactivities is still ugently needed. Glycosaminoglycans (GAGs) are important complex carbohydrates that participate in many biological processes through the regulation of their various protein partners. Biochemical, structural biology and molecular modelling approaches have assisted in understanding the molecular basis of such interactions, creating an opportunity to capitalize on.

collagen, and proteoglycans with their associated glycosaminoglycans (GAG). While it is generally believed that relationships exist between tendon fibrillar structure, composition and mechanical properties, little quantitative data exists to formally support these beliefs. The availabl Glycosaminoglycans (linear polysaccharides with a repeating disaccharide backbone containing an amino sugar) are essential components of extracellular matrices of animals. As these enzymes have only recently been discovered, their complete structure/function relationships are not known. A single gene, and thus a single protein, is thought. noncovalent interacting partners and their structure-function relationship should provide important information for possible therapeutic intervention12. However, owing to their structural complexity, the characterization of the structure-function rela--mous structural diversity Glycosaminoglycans (GAGs) possess considerable heterogeneity in. Optimized extraction of glycosaminoglycans from normal and osteoarthritic cartilage for glycomics profiling. Glycobiology 2007, 17 (1) , 25-35. DOI: 10.1093/glycob/cwl046. Ram Sasisekharan, Rahul Raman, Vikas Prabhakar. GLYCOMICS APPROACH TO STRUCTURE-FUNCTION RELATIONSHIPS OF GLYCOSAMINOGLYCANS

structure-function relationships of this class of carbohydrates, and in particular, understand the fine structural features which govern their specificity of interactions. Measurement of acidic glycans, however, by typical analytical methods, even those that have proven usefu Structure-function relationships of deep eutectic solvents for lignin extraction and chemical transformation overview of important studies to provide insights into lignin extraction and chemical transformations by examining the relationship between the type and number of functional groups in DES constituents during pretreatment.

Glycosaminoglycans as polyelectrolytes Emek Seyreka, Paul Dubinb,⁎ a CNRS, Insitut Charles Sadron, 23 Rue Loess, BP 84047, F-67037 Strasbourg 2, France b University of Massachusetts, Department of Chemistry, Amherst, MA 01003 USA article info abstract Available online 20 March 2010 One of the barriers to understanding structure-property relations for glycosaminoglycans has been the lac Glycosaminoglycans are commonly found attached to proteins and these are referred to as proteoglycans. Linkage between the protein and the glycosaminoglycan is made through a serine side-chain. Proteoglycans are made by glycosylation of target proteins in the Golgi apparatus. Back to top; 2.6: Structure and Function - Nucleic Acid CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Understanding structure function relationships in the temporomandibular joint (TMJ) disc is a critical first step toward creating functional tissue replacements for the large population of patients suffering from TMJ disc disorders. While many of these relationships have been identified for the collagenous fraction of.

Glycosaminoglycan - Wikipedi

Lipoprotein (a) [Lp(a)] has attracted the interest of researchers and physicians due to its intriguing properties, including an intragenic multiallelic copy number variation in the LPA gene and the strong association with coronary heart disease (CHD). This review summarizes present knowledge of the structure, function, and genetics of Lp(a) with emphasis on the molecular and population. Hence, comprehensive structure-function relationships that consider the replete proteoglycan architecture as glycoconjugates are limited. Here, we present a comprehensive approach to study proteoglycan structure and biology by fabricating defined semi-synthetic modular proteoglycans that can be tailored for cell surface display Abstract. Basic fibroblast growth factor (bFGF) belongs to the family of the heparin-binding growth factors which includes also acidic FGF and five other gene products (Basilico and Moscatelli, 1992). bFGF exerts various biological activities in vitro and in vivo on different cell types. In particular, bFGF is an angiogenic molecule that induces a set of complex, coordinated responses in.

Cells respond to changes in mechanical strains by varying their production of extracellular matrix macromolecules. Because differences in strain patterns between mitral valve leaflets and chordae tendineae have been linked to different quantities and types of glycosaminoglycans (GAGs), we investigated the effects of various strain conditions on GAG synthesis by valvular interstitial cells. Two cases studies, one involving sialylated branched glycans and the other sulfated glycosaminoglycans, are used to highlight how integration of orthogonal information from diverse datasets enables rapid convergence of glycan characterization for development of robust structure-function relationships My research focus is the structure/function relationships of glycosaminoglycans (GAGs) and the role of the microenvironment in directing cell behaviour. Since 2006 I have developed a biomaterials expertise, primarily as a way of displaying GAGs to cells and to generate artificial microenvironments - a key priority for regenerative medicine

ratios (0:4:6-sulfation) of the chondroitin sulfate glycosaminoglycans (GAGs) that decorate its protein backbone [1,2]. The goal of the present study was to develop and validate a computationally efficient, atomistic-based model of chondroitin sulfate that may be used to investigate the relationship between the chemical composition and the physico The alternative splicing of the cxd12 gene generates a recently identified isoform, CXCL12γ, which structure/function relationships remain unexplored. The high occurrence of basic residues that characterize this isoform suggests however that it could feature specific regulation by HS. Methodology/Principal Findings Free glycosaminoglycans (GAGs) and proteoglycan- (PG-) containing GAGs, key effectors of cell surface, pericellular and extracellular microenvironments, perform multiple functions in cancer by virtue of their coded structure and their ability to interact with both ligands and receptors that regulate cancer growth [ 1

Heparin, the drug of choice for the prevention and treatment of thromboembolic disorders, has been shown to interact with many proteins. Despite its widespread medical use, little is known about the precise sequences that interact with specific proteins. The minimum heparin binding sequence for FGF1 and FGF2 necessary to promote signaling was investigated. A characteristic pentasaccharide. Glycosaminoglycans (GAGs), the polysaccharide component of the extracellular matrix (ECM), are the most acidic naturally occurring biopolymers. These GAGs not only hydrate the ECM, but also solubilize several transient molecules, such as growth factors, cytokines, enzymes etc. that diffuse from the outside of a tissue to the cell surface to.

Video: Glycosaminoglycan-mediated leuserpin-2/thrombin

Glycosaminoglycans, or GAGs, are long-chain polysaccharides ('poly' meaning 'many,' and 'saccharide' meaning 'sugar').There are several classes of GAGs whose chemical properties are determined by. Heparin and heparan sulfate (Hp/HS) are linear complex glycosaminoglycans which are involved in diverse biological processes. The structural complexity brings difficulties in separation, making the study of structure-function relationships challenging Proteoglycans have emerged as biomacromolecules with important roles in matrix remodeling, homeostasis, and signaling in the past two decades. Due to their negatively charged glycosaminoglycan chains as well as distinct core protein structures, they interact with a variety of molecules, including matrix proteins, growth factors, cytokines and chemokines, pathogens, and enzymes. This led to the. The glycosaminoglycans and proteoglycans are highly hydrophilic. The tissue fluid within the meshes permits the diffusion of nutrients and metabolites between the connective tissue cells and circulatory system. The gel-like nature of the hyaluronic acid is thought to act as a barrier against the spread of bacteria that may enter the tissues Sasisekharan, Ram; Raman, Rahul; Prabhakar, Vikas (August 2006). Glycomics Approach to Structure-Function Relationships of Glycosaminoglycans